Organic & Biochemistry
  • UV-visible spectroscopy – advancements in experimental methods, analysis of mixtures, dissociation constants of acids and bases, study of enzyme catalysis, applications of ORD and CD.
  • IR spectroscopy – intra and intermolecular hydrogen bonding, effect of concentration and temperature, Fourier transform IR, applications of UV, VISIBLE AND IR in the structural elucidation of complex natural products.
  • NMR spectroscopy – experimental aspects, FT NMR, factors influencing sensitivity and resolution,applications of chemical shift and spin-spin coupling, Karplus equation and conformational analysis; NMR of carbon-13, fluorinee-19, phosphorous-31, Nitrogen- 14 and 15 and oxygen 17. Multiple-pulse experiments – gated decoupling and NOE, measurement of T1 and T2, polarisation transfer, INEPT, DEPT, two – dimensional NMR, J-resolved spectra, shift correlated spectra including COSY, HETCOR, INADEQUATE, NOE correlted spectroscopy, applications of 2D-NMR in the structural analysis of complex natural products and biopolymers, applications of solid state NMR.
  • EPR Spectroscopy – epr spectra of solutions, frozen solution, especially organic molecules.
  • Mass spectrometry – high resolution mass spectrometry, linked scans, MIKES, HV scan, negative ion mass spectrometry, applications of field desorption, plasma desorption, fast atom bombardment, electrospray and tandem mass spectrometry and FTMS.
  • Also included are topics from current literature on the applications of modern spectroscopic techniques.


  1. C.N.R. Rao, UV and Visible spectroscopy, Butterworths, 3rd edition, London, 1972
  2. K. Nakanishi, Infrared absorption spectroscopy – practical, Holden-Day, Inc., San Francisco and Nankodo Company Ltd., Tokyo, 1962.
  3. J.K.M. Sanders and B.K,. Hunter, Modern NRM Spectroscopy: A Guide for chemists, Oxford University Press, London, 1987.
  4. W. Kemp, NMR in Chemistry, A Multinuclear Introduction, McMillan, London, 1986.
  5. W.R. Croasmun and R.M.K. Carlson (Ed), Two-dimensional NMR spectroscopy, Applications for Chemists and Biochemists, VCH, New York, 1987.
  6. G.W. Milane, Mass spectrometry: Techniques and Applications, wiley- Interscience, New York, 1971.
  7. Q.N. Porter, Mass Spectrometry of Heterocyclic Compounds , Wiley- Interscience, New York, 1985.
  8. F.W. McLafferty, Interpretation of Mass Spectra, 3rd Edition, Mill Valley, CA University Co. Books, 1980.
  9. F.W. McLfferty and R. Venkataraghavan, Mass Spectral Correlations, American Chemical Society , Washington D.C., 1982.
  10. J.R. Chapman, Practical Organic Mass Spectrometry, Wiley, New York, 1985
  11. H.D. Beckly, Principles of Field Desorption and Field Ionisation Mass Spectrometry Pergamon Press, New York, 1977.
  12. R.S. Drago, Physical methods in Inorganic Chemistry, 2nd Edn., Chapman and Hall, 1977.