CY5020 : Analytical Chemistry: Principles, Practices and Applications

Course Objectives: The learners should be able to apply the conceptual understanding of the principles and implementation modes of several analytical instruments to chemical systems.

Learning Outcomes: At the end of the course, the learners should be able to:

Solve problems based on various analytical concepts

Design experiments with improved sample preparation, new measurement procedures and tools

Quantify analytes with proper data handling and analysis Design sensors

Course Contents:

Historical overview and the current status of analytical chemistry: an introduction.

Statistics for analytical experimentation: Probability, Regression analysis, Accuracy and propagation of errors, Data analysis and signal enhancement.

Advanced chromatographic techniques: Theory of separation methods: HPLC, GC, GC/MS, LC/MS, GPC, Supercritical fluid chromatography, Detectors in Chromatography, Applications of chromatography

Electroanalytical techniques: Applications to chemical & biological systems: Principles of Potentiometry, Electrogravimetry, Voltammetry, Stripping methods, Chronoamperometry, Quantitative applications of Potentiometry and Voltammetry: Electrochemical sensors, ISFETs, CHEMFETs.

Spectrometric and Spectroscopic methods: Acid-base equilibria, Methodology in spectrochemical analysis, Spectrophotometry and binding assays. Introduction to electromagnetic radiation, Optical components of a spectrometer, Sources (LASERS), Detectors. Atomic absorption and emission spectroscopy, Principles and applications of Fluorimetry, Dynamic light scattering. Preliminary analyses of a spectrum: Relative populations of species from intensity, Relate line widths to lifetime, Introduction to spectroscopy in time domain, Time-correlated single photon counting.

Physical methods of characterization: Surface Techniques: Principles and applications of electron spectroscopy for chemical analysis (ESCA) and Scanning Probe Microscopy.

Text Books:

1. A. Skoog, F. J. Holler and S. R. Crouch, Principles of Instrumental Analysis, 6th Edition, Brooks/Cole Cengage Learning, Belmont, CA, 2007
2. H. Willard, L. L. Merrln, Jr., J. A. Dean, and F. A. Senle, Jr., Instrumental Methods of Analysis: Wadsworth, 7th Edition, Belmont., 1989
3. Rousseac and A. Roessac, Chemical Analysis: Modern Instrumentation Methods and Analysis, 4th Edition, John Wiley & Sons, Ltd., 2000
4. Wang, Analytical Electrochemistry, 3rd Edition, Wiley – VCH, 2006
5. T. Kissinger and W. R. Heineman, Laboratory Techniques in Electroanalytical Chemistry, 2nd Edition, Marcel Dekker Inc., 1996
6. Voigtlaender, Scanning Probe Microscopy: Atomic Force Microscopy and Scanning Tunneling Microscopy:, Springer – Verlag, Berlin 2015