DEPARTMENT OF CHEMISTRY
INDIAN INSTITUTE OF TECHNOLOGY MADRAS

DEPARTMENT OF CHEMISTRY
INDIAN INSTITUTE OF TECHNOLOGY MADRAS

CY7120 : Bioanalytical Techniques

Organic & Biochemistry
  • pH and buffers used in biochemical experiements, pKa values and titration curves.
  • Determination of biomolecules by absorption photometry, fluorimetry, biochemical applications of mass spectrometry.
  • Manometry and respiration measurement, oxygen polarography, BOD estimation, resting cells prepastion.
  • Principles of microscopy – light, darkfield, phase contrast, fluorescence and eletron microscope, fixing and preparation of samples.
  • Breaking of cells by chemical and physical methods, ultrasonication, pressure cell disintegrators, detection of cell-free and cell-bound proteins.
  • Principles of centrifugation – calculation of r.c.f. values, ultracentrifuges, density gradients.
  • Enzyme assays.
  • Biomolecules separation by chromatography, paper, thin layer and column chromatrography, ion exchangers, molecular sieves, affinity columns, Gas chromatography and HPLC.
  • Electrophoresis – theory and practice, mobility, buffers, agarose and polyacrylamide matrices, gel apparatuses, native and SDS -PAGE gels, Isoelectric focussing, Zymograms.
  • Immunochemical methods – immunoassays, immunodiffusion, rocket immunoelectrophoresis.
  • Use of radioisotope tracer techniques in biochemical experiments and their detection.

References:

  1. Scopes R, Protein purification : Principles and practice, Springer-Verlag NY 1982
  2. Plummer D.T., An introduction to practical biochemistry, 3rd edition, Tata McGraw Hill, 1988
  3. P.Gerhardt (Ed.), Methods for General Bacteriology, Amer.Soc. Microbiol/ Washington, 1981.
  4. N.C. Price and L. Stevens, Fundamentals of Enzymology, Oxford University Press, 1989.
  5. Protein structure: a practical approach, (Ed) T.E. Creighton, IRL Press, 1989.
  6. S. Narayanan, Principles and applications of Laboratroy Instrumentation, ASCP Press 1989.
  7. D.J. Holme and H.Peck, Analytical Biochemistry, Longman Group, 1983.
  8. E.A. Dawes, Quantitative problems in biochemistry, 6th edition, Longman, 1978.
  9. A biologist’s guid e to principles and techniques of prctical biochemistry, (Ed) K. Wilson and K.H. Goulding, 3rd edition, Edward Arnold Publ., 1986.
  10. Protein purification methods – a practical approach, (Ed) E.L.N.Harris and S. Angal, IRL Press, 1989.
  11. R.F. Schleif, and P.C. Wensink, Practical Methods in Molecular Biology, Springer Verlag 1981.

 

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