DEPARTMENT OF CHEMISTRY
INDIAN INSTITUTE OF TECHNOLOGY MADRAS

Q-Tof-Mass Spectometer

The Q-Tof micro hybrid quadrupole time of flight mass spectrometer is available with electrospray ionisation (ESI) and atmospheric pressure chemical ionisation (APcI).

Q-Tof micro utilises a high performance, research grade quadrupole mass analyser, incorporating a prefilter assembly to protect the main analyser from contaminating deposits, and an orthogonal acceleration time of flight (TOF) mass spectrometer. A hexapole collision cell, between the two mass analysers, can be used to induce fragmentation to assist in structural investigations.

Ions emerging from the second mass analyser are detected by the microchannel plate detector and ion counting system

Exact mass MS/MS is achieved automatically using the Q-Tof�s LockSpray� dual electrospray source. The LockSpray dual electrospray source enables automated exact mass measurement with an infused internal lockmass from a second sprayer, eliminating the need for T-plumbing and potential ionization interference between analytes and standard. The NanoLockSpray dual electrospray source is optimized for exact mass data acquisition with nanoflow LC/MS/MS and is ideal for proteomics studies or low level metabolite identification.

A PC computer runs the MassLynx NT software system to control Q-Tof micro, and to acquire and process data.