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DEPARTMENT OF CHEMISTRY
INDIAN INSTITUTE OF TECHNOLOGY MADRAS
CY7320 : ADVANCED SPECTROSCOPIC TECHNIQUES IN ORGANIC CHEMISTRY AND BIOCHEMISTRY
Organic & Biochemistry
- UV-visible spectroscopy – advancements in experimental methods, analysis of mixtures, dissociation constants of acids and bases, study of enzyme catalysis, applications of ORD and CD.
- IR spectroscopy – intra and intermolecular hydrogen bonding, effect of concentration and temperature, Fourier transform IR, applications of UV, VISIBLE AND IR in the structural elucidation of complex natural products.
- NMR spectroscopy – experimental aspects, FT NMR, factors influencing sensitivity and resolution,applications of chemical shift and spin-spin coupling, Karplus equation and conformational analysis; NMR of carbon-13, fluorinee-19, phosphorous-31, Nitrogen- 14 and 15 and oxygen 17. Multiple-pulse experiments – gated decoupling and NOE, measurement of T1 and T2, polarisation transfer, INEPT, DEPT, two – dimensional NMR, J-resolved spectra, shift correlated spectra including COSY, HETCOR, INADEQUATE, NOE correlted spectroscopy, applications of 2D-NMR in the structural analysis of complex natural products and biopolymers, applications of solid state NMR.
- EPR Spectroscopy – epr spectra of solutions, frozen solution, especially organic molecules.
- Mass spectrometry – high resolution mass spectrometry, linked scans, MIKES, HV scan, negative ion mass spectrometry, applications of field desorption, plasma desorption, fast atom bombardment, electrospray and tandem mass spectrometry and FTMS.
- Also included are topics from current literature on the applications of modern spectroscopic techniques.
References:
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C.N.R. Rao, UV and Visible spectroscopy, Butterworths, 3rd edition, London, 1972
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K. Nakanishi, Infrared absorption spectroscopy – practical, Holden-Day, Inc., San Francisco and Nankodo Company Ltd., Tokyo, 1962.
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J.K.M. Sanders and B.K,. Hunter, Modern NRM Spectroscopy: A Guide for chemists, Oxford University Press, London, 1987.
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W. Kemp, NMR in Chemistry, A Multinuclear Introduction, McMillan, London, 1986.
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W.R. Croasmun and R.M.K. Carlson (Ed), Two-dimensional NMR spectroscopy, Applications for Chemists and Biochemists, VCH, New York, 1987.
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G.W. Milane, Mass spectrometry: Techniques and Applications, wiley- Interscience, New York, 1971.
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Q.N. Porter, Mass Spectrometry of Heterocyclic Compounds , Wiley- Interscience, New York, 1985.
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F.W. McLafferty, Interpretation of Mass Spectra, 3rd Edition, Mill Valley, CA University Co. Books, 1980.
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F.W. McLfferty and R. Venkataraghavan, Mass Spectral Correlations, American Chemical Society , Washington D.C., 1982.
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J.R. Chapman, Practical Organic Mass Spectrometry, Wiley, New York, 1985
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H.D. Beckly, Principles of Field Desorption and Field Ionisation Mass Spectrometry Pergamon Press, New York, 1977.
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R.S. Drago, Physical methods in Inorganic Chemistry, 2nd Edn., Chapman and Hall, 1977.
