Overview of basic concepts: Light-matter interaction, Einstein coefficients, introduction to lasers, transition dipole moment, selection rules for electronic transitions, Jablonskii diagram, fluorescence and phosphorescence, kinetics of unimolecular and bimolecular processes.
Advanced concepts: Theory of nonradiative transitions, spin-orbit coupling and singlet-triplet transitions, polarized light absorption and emission: fluorescence anisotropy, solvation dynamics, energetics and dynamics of bimolecular processes like excimer and exciplex formation, resonance energy transfer, mechanisms of fluorescence quenching, introduction to non-linear spectroscopy.
Techniques and instrumentation: Uv-Vis spectrophotometry, steady-state fluorimetry, lasers as excitation sources, time-resolved fluorimetry, transient absorption spectroscopy, surface plasmon spectroscopy, evanescent wave spectroscopy, multiphoton spectroscopy, single-molecule spectroscopy, fluorescence correlation spectroscopy.
Applications: Microscopy (optical, phase-contrast, confocal, FLIM). Applications in biology and analytical chemistry.