Course Objectives: The learners should be able to apply the conceptual understanding of the principles and implementation modes of several analytical instruments to chemical systems.
Learning Outcomes: At the end of the course, the learners should be able to:
Solve problems based on various analytical concepts
Design experiments with improved sample preparation, new measurement procedures and tools
Quantify analytes with proper data handling and analysis Design sensors
Historical overview and the current status of analytical chemistry: an introduction.
Statistics for analytical experimentation: Probability, Regression analysis, Accuracy and propagation of errors, Data analysis and signal enhancement.
Advanced chromatographic techniques: Theory of separation methods: HPLC, GC, GC/MS, LC/MS, GPC, Supercritical fluid chromatography, Detectors in Chromatography, Applications of chromatography
Electroanalytical techniques: Applications to chemical & biological systems: Principles of Potentiometry, Electrogravimetry, Voltammetry, Stripping methods, Chronoamperometry, Quantitative applications of Potentiometry and Voltammetry: Electrochemical sensors, ISFETs, CHEMFETs.
Spectrometric and Spectroscopic methods: Acid-base equilibria, Methodology in spectrochemical analysis, Spectrophotometry and binding assays. Introduction to electromagnetic radiation, Optical components of a spectrometer, Sources (LASERS), Detectors. Atomic absorption and emission spectroscopy, Principles and applications of Fluorimetry, Dynamic light scattering. Preliminary analyses of a spectrum: Relative populations of species from intensity, Relate line widths to lifetime, Introduction to spectroscopy in time domain, Time-correlated single photon counting.
Physical methods of characterization: Surface Techniques: Principles and applications of electron spectroscopy for chemical analysis (ESCA) and Scanning Probe Microscopy.